• Physicochemistry
- Vapor treatment and extraction(solvent,CO²)
- Moisture(Karl Fisher, azeotropic)
- Physicochemical Indices
- Density
- Chemical Indices
- Etc.


• Gas chromatography
- Profiles and percentages (HPLC UV, DAD)
- Coupling HPLC/MS
- Quantitative determination
- Chiral Analysis
- Dynamic and static Headspace
- SPME


• Chromatography in liquid phase
- Relative Profiles and percentages
- Coupling HPLC/SM
- Quantitative Proportioning
- Chiral Analysis

LEXVA Analytique benefits from an experiment on analytical methods of Essential Oils, which is recognized in this field thanks to the publication of more than 200 publications in international papers.

The laboratory has a bank of reference of chromatograms of more than 400 different essential oils and proposes with its customers the following analyses:

• Physicochemistry according to standards' AFNOR and Pharmacopeia
  - Index of refraction
  - Density
  - Optical Activity
  - Solubility
  - Acid value
  - Ester Index
  - Peroxide Index
  
• Gas chromatography (CPG)
  - Profiles and percentages
  - Composition in relative percentages
  - Quantitative determination (curve calibration 4 points)
  - Allergens, CMR
  - Undesirable Products (phthalates, residue solvent, etc.)
  - Chiral Analysis
  

Chromatogram of an essential oil of Madagascar black pepper :

Move the mouse on the chromatogram to obtain more details on the molecules such as their spectrum of mass.

Alpha-pinène Retention time : 10.18 min Kovat index : 939

Béta-pinène Retention time : 11.75 min Kovat index : 980

Myrcène Retention time : 12.14 min Kovat index : 991

Delta-3-carène Retention time : 12.82 min Kovat index : 1011

Limonène Retention time : 13.54 min Kovat index : 1031

Delta-élémène Retention time : 22.63 min Kovat index : 1339

Béta-caryophyllène Retention time : 24.90 min Kovat index : 1418

Germacrène-D Retention time : 26.37 min Kovat index : 1480

Alpha-phellandrene Retention time : 12.73 min Kovat index : 1005

Béta-élémène Retention time : 24.05 min Kovat index : 1391

Alpha-humulène Retention time : 25.75 min Kovat index : 1454

Béta-sélinène Retention time : 26.58 min Kovat index : 1485

Alpha-sélinène Retention time : 26.73 min Kovat index : 1494

Oxyde de caryophyllène Retention time : 28.77 min Kovat index : 1581

• Physicochemistry
  - Fat content Extraction
  - Content of insoluble impurities
  - Measurement of alkalinity
  - Peroxide Index
  - Acid value
  - Iodine Index
  - Index of saponification
  - Hydroxyl Index
  - Content of insaponifiable
  - Index of refraction
  
  
• Gas chromatography
  - Analysis of methyl esters :relative profiles and percentages
  - Coupling CPG/MS
  - Quantitative determination
  - Assay of the fatty acids
  - Assay of insaponifiable(sterols, terpenes, etc.)
  - Vitamin E,etc.
  

Chromatogram of a vegetable oil of shea butter:

Move the mouse on the chromatogram to obtain more details on the molecules such as their spectrum of mass.

Palmitate de méthyle Retention time : 23.72 min

Stéarate de méthyle Retention time : 27.01 min

Oléate de méthyle Retention time : 28.00 min

Linoléate de méthyle Retention time : 29.42 min

Arachidate de méthyle Retention time : 29.97 min

• Physicochemistry
  - Soxlhet Extraction
  - Moisture
  - Ashes
  - Quantification of specific tracers:
              alcaloïdes : yohimbine
              phytosterol : ß sitosterol
  - Isolation and purification of compounds